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A Novel Cytogenetic Variant Translocation in Acute Myeloid Leukemia
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Monika V Patel1, Salil N Vaniawala2, Pankaj K Gadhia2 1,2JJTU University, Rajasthan, India; 2Molecular Cytogenetic Unit, S.N. Gene Laboratory and Research Centre, Surat, India. |
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Corresponding Author:
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Dr. Pankaj K. Gadhia Email: pankajkgadhia@gmail.com |
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Received:
12-JUN-2017 |
Accepted:
10-JUL-2017 |
Published Online:
30-JUL-2017 |
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Abstract
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Background: Acute myeloid leukemia (AML) is a heterogeneous group of malignant neoplasm of hematopoietic disorders characterized by an abnormal proliferation of myeloid precursors. Variant forms of classic translocation t(8;21) are uncommon, and either third or fourth chromosomes accounts for approximately 3-4% in acute myeloid leukemia. Case Report: We report a rare case of a 16-year old male having t(8;19;21)(q22;q13;q22) associated with loss of sex chromosome. To the best of our knowledge, only few cases are described with variants of t(8;21) in AML and we first time report the involvement of 19q13 breakpoint region in AML. Conclusion: Translocation t(8;19;21)(q22;q13;q22) is a novel, rare but recurrent variant translocation of t(8;21). Due to the rarity of this translocation future accumulation of similar cases needed to evaluate clinical as well as prognostic and biological features. |
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Keywords :
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Chromosomes, Cytogenetics, Karyotyping, Neoplasms, Sex Chromosome, Translocation.
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6go6ckt5b8|3000F7576AC3|Tab_Articles|Fulltext|0xf1ffecd71a0000003006000001000100 Venlafaxin Ohne RezeptAntidepressants are medications prescribed to treat mental health conditions such as depression, anxiety, obsessive-compulsive disorder, and post-traumatic stress disorder. In Germany, these medications are only available with a prescription from a licensed healthcare provider. It is common to feel overwhelmed and scared when dealing with mental health issues, but it is important to know that effective treatment options are available. Antidepressants can be a valuable part of a comprehensive treatment plan, alongside therapy and other types of support. Collaborating with a healthcare provider is essential to determine whether antidepressants are appropriate for your specific condition and to find the right medication and dosage for you. Antidepressants may take some time to work, so it is important to give them time to take effect before adjusting your treatment plan. Like all medications, antidepressants can cause side effects. However, many people can take them without experiencing significant problems. Some common side effects of antidepressants include nausea, dry mouth, and changes in appetite or sleep patterns. While more serious side effects are rare, they can include suicidal Venlafaxin Ohne Rezept thoughts or behavior. If you are struggling with depression or another mental health condition in Germany, remember that you are not alone. Speaking with a licensed healthcare provider about whether antidepressants may be suitable for you is an important first step towards a brighter and healthier future. 6go6ckt5b5idvals|776 6go6ckt5b5|2000F757Tab_Articles|Fulltext Introduction
Acute myeloid leukemia (AML) is a heterogeneous group of malignant neoplasm of hematopoietic disorders characterized by an abnormal proli-feration of myeloid precursors [ 1]. Conventional cytogenetic analysis can be used for identification recurrent chromosomal abnormality and thereby it serves as one of the most important diagnostic and prognostic markers in AML patients. Variants of t(8;21) involving chromosome 8,21 and other chromosomes account for approximately 3-4% in AML associated t(8;21) [ 2, 3].
The translocation t(8;21)(q22;q22) is recurrent chromosomal aberrations typically found in AML and is closely associated with French American British (FAB) classification M2 subtype and less frequently in M1 or M4 subtype [ 4]. In addition, the complex cytogenetic variants of this translocation are the three-way translocations involving regions of 8q22, 21q22 and third chromosome [ 5]. Molecularly, the t(8;21) leads to fusion of AML1 (RUNX1) gene located at 21q22 to ETO(RUNX1T1) gene located at 8q22, generating a chimeric AML1/ETO fusion gene on derivative chromosome 8 (der8) [ 2]. However, Grimwade et al. [ 6] suggested that the patients positive for this chimeric fusion are known to have a favorable prognosis. In addition, the formation of AML1/ETO chimeric fusion gene are frequently accompanied by additional chromosomal changes such as loss of sex chromosome and del(9) (q22) [ 7]. The frequency is approximately 10-15% of all such cases [ 8].
Moreover, the variants in some cases present as hidden translocation, in such cases it is difficult to identify the t(8;21) by conventional cytogenetic analysis [ 4]. Here, we describe a case of three-way complex translocation t t(8;19;21)(q22;q13;q22) associated with additional chromosomal abnormalities including loss of Y chromosome.
Case Report
A 16 years old male with no significant past medical history was referred for cytogenetic analysis. His complete blood counts showed a hemoglobin level 4.4 g/dL, a platelet count of 97000/µL and white blood cell count of 8400/µL with 37.0% neutrophils, 18.0% lymphocyte, and 45% blasts. The initial results of the karyotype of this patient were 45, X,-Y,t(8;19;21)(q22;q13;q22) in all metaphases analyzed.
Conventional cytogenetic technique was performed on 24 hours unstimulated short term culture of bone marrow cells. The cells were grown in culture medium Marrowmax (GIBCO) supplemented with 20% of FBS (fetal bovine serum). The colcemid was added for 30 minutes followed by KCL (75mM) at room temperature for 27 minutes and Carnoys fixative was used to fix the cells for four times. Slides were stained with GTG banding technique [ 9]. GTG banded metaphase from each culture was analyzed and karyotyped by using automatic IKAROS karyotyping software. In general, twenty metaphases from each specimen were analyzed as per the International System for Human Cytogenetic Nomenclature (ISCN) [ 10].
A dual colour FISH assay using Abbott Molecular Vysis Locus-specific identifier Eto and AML1 specific probes was performed on bone marrow cells. The ETO (RUNX1T1) was directly labeled with spectrum orange and RUNX1 (AML1) with spectrum green. The slides were hybridized overnight and subsequently analyzed. Microscopic evaluation of metaphases chromosome revealed a reciprocal translocation between 8q22,19q13 and 21q22. Cytogenetic analysis of BM cells revealed an abnormal karyotype with a novel complex translocation involving chromosome 8,21,19 with the loss of Y chromosome, resulting in the karyotype 45, X,-Y,t(8;19;21)(q22;q13;q22) in all metaphases analyzed [Fig.1].
FISH analysis results showed that the cells had one orange signal corresponding to RUNX1T1(ETO) gene on chromosome 8, one green corresponding to RUNX1(AML1) gene on chromosome 21 and two yellow, orange, green fusion RUNX1T1/RUNX1 signals located on der(8) and der(21) [Fig.2].
Discussion
Complex cytogenetic variants of the classic translocation are uncommon in AML and it can exist in a small percentage of AML patients [ 5]. However, the t(8;21)(q22;q22) which creates AML1/ETO fusion gene is a distinct type of transcript generally associated with favorable prognosis in the AMLM2 patient [ 1].
Brahmbutt et al. [ 11], suggested that the variants of t(9;22) were observed in 5% cases of CML. Whereas, in AML variants of translocation t(8;21) were observed in 3-4% of cases involving chromosome 1,2,4,5,6,7,8,10,12,13,15,17,18,19 or 20 [ 12, 13]. But the prognosis of this variants is contraversial [ 14]. However, Liu et al. [ 15], suggested that the there was no obvious differences between complex and typical t(8;21) with regards to remission rate and disease-free survival whereas, Gong et al. [ 16], suggested that the additional chromosome involved in the translocation could change the biological characteristic of the leukemic cells. Although the involvement of chromosome 19 with t(8;21) has been reported previously [ 7], the case reported here differs from previous reports in certain respects [ 17]. Interestingly this study reported by the Park et al. [ 7], suggested that the only two cases of three-way variants of t(8;21) involving chromosome 19 have been reported in AML as complex translocation which contains 8q22, 19p13, 21q22 region. They have also suggested that 19q13 breakpoint region was not reported previously but in the present study 19q13 breakpoint region in AML was found.
Furthermore, Farra et al. [ 2] pointed out that the t(8;21) are reportedly associated with additional chromosomal abnormalities such as sex chromosome loss, trisomy 8 and structural aberrations involving 9q. The determination of the significance of variants is important because to assess the prognosis in terms of therapy outcome.
Conclusion
In conclusion, our study indicated that the conventional cytogenetic investigation is important for the rapid and precise diagnosis of t(8;21). Translocation t(8;19;21)(q22;q13;q22) is a novel, rare but recurrent variant translocation of t(8;21). Due to the rarity of this translocation, future studies dealing with t(8;21) variants are needed to evaluate clinical as well as prognostic and biological features and its significance.
Contributors: MVP: manuscript editing, and literature search; SNV: manuscript writing; PKG: critical inputs into the manuscript and experimental process. PKG will act as guarantor. All authors approved the final version of the manuscript. Funding: None; Competing interests: None stated.
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Patel MV, Vaniawala SN, Gadhia PKA Novel Cytogenetic Variant Translocation in Acute Myeloid Leukemia.JCR 2017;7:270-273 |
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Patel MV, Vaniawala SN, Gadhia PKA Novel Cytogenetic Variant Translocation in Acute Myeloid Leukemia.JCR [serial online] 2017[cited 2024 Apr 26];7:270-273. Available from: http://www.casereports.in/articles/7/3/A-Novel-Cytogenetic-Variant-Translocation-in-Acute-Myeloid-Leukemia.html |
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