Background: Conventional treatments of osteoarthritis demonstrate only modest clinical benefits without lesion reversal. Autologous mesenchymal stem cells (MSCs) are known to have potential for articular cartilage regeneration. We assess the feasibility and safety of treating osteoarthritis autologous MSCs in combination with platelet rich plasma (PRP). Material & Methods: Patients with chronic knee pain unresponsive to conservative treatments and showing radiological evidence of osteoarthritis were enrolled and injected adipose derived mesenchymal stem cells intra-articularly. The MSC-treated patients displayed significant improvement with Western Ontario and McMaster Universities Osteoarthritis index (WOMAC) score. No adverse events related local or systemic were found. Conclusion: Autologous MSC therapy may be a valid alternative for the treatment of chronic knee osteoarthritis.